Synthesis and characterisation of lipase enzyme immobilised on magnetic nano particles for application in the transesterification of soybean oil to biodiesel
Abstract
In the study, magnetic chitosan microspheres were prepared by the chemical co-precipitation approach using glutaraldehyde as the cross-linking reagent for lipase immobilisation. The lipase enzyme is immobilised on the principle of creating peptides covalently linked via glutaraldehyde as a bridge with chitosan magnetic particles. The catalyst
system has been characterised using the following analytical methods: X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectra, and transmission electron microscopy (TEM). The analysis showed that the enzyme was immobilised onto the magnetic nanoparticles. The nanoparticles formed were spherical, uniform in size with average diameters from 12 - 15nm. Using the immobilised lipase, the conversion of soybean oil to fatty acid methyl esters reached 93%wt under the reaction temperature of 40oC and reaction duration of 30h, and methanol/oil amount-of-substance ratio of 4:1 with the three-step addition of methanol.
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